Caldalkalibacillus salinus sp. nov., isolated from a salt lake in Xinjiang, northwest China.

A novel Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated as strain YIM B00319, was isolated from a sediment sample obtained from Wuzunbulake salt Lake in Xinjiang Uygur Autonomous Region, northwest China. Phylogenetic analysis based on 16S rRNA gene sequences along with the whole genome showed that strain YIM B00319 belongs to the family Bacillaceae and was most closely related to Bacillus horti K13 and Caldalkalibacillus mannanilyticus JCM 10596, with sequence similarities of 95.7% and 94.6%, respectively. The genome of strain YIM B00319 was 3.77 Mbp with a DNA G + C content of 43%. Strain YIM B00319 grew at 15-45 ℃, pH 7.0-9.5 and with 3-11% (w/v) NaCl. The major respiratory quinone of strain YIM B00319 was MK-7, and the major fatty acids (> 10%) were iso-C, anteiso-C, and summed feature 3 (C ω6c and/or C ω7c). The main polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and diphosphatidylglycerol (DPG). The cell-wall peptidoglycan contained meso-diaminopimelic acid. On the basis of the phenotypic, chemotaxonomic, genomic, and phylogenetic information, strain YIM B00319 represents a novel species of the genus Caldalkalibacillus, for which the name Caldalkalibacillus salinus sp. nov. is proposed. The type strain is YIM B00319 (= CGMCC 1.18750 = NBRC 115338).