AI Article Synopsis
- - Spatial chromatin organization is essential for how genes are regulated in neurons, especially since these cells can change their gene expression when stimulated.
- - Neuronal stimulation triggers the condensation of large chromatin domains quickly and reversibly, relying on energy and calcium pathways, but not on active transcription.
- - The process involves changes in histone modifications and the spatial arrangement of chromosomes, with histone deacetylase HDAC1 playing a crucial role in this chromatin reorganization, affecting transcriptional regulation.
Article Abstract
Spatial chromatin organization is crucial for transcriptional regulation and might be particularly important in neurons since they dramatically change their transcriptome in response to external stimuli. We show that stimulation of neurons causes condensation of large chromatin domains. This phenomenon can be observed in vitro in cultured rat hippocampal neurons as well as in vivo in the amygdala and hippocampal neurons. Activity-induced chromatin condensation is an active, rapid, energy-dependent, and reversible process. It involves calcium-dependent pathways but is independent of active transcription. It is accompanied by the redistribution of posttranslational histone modifications and rearrangements in the spatial organization of chromosome territories. Moreover, it leads to the reorganization of nuclear speckles and active domains located in their proximity. Finally, we find that the histone deacetylase HDAC1 is the key regulator of this process. Our results suggest that HDAC1-dependent chromatin reorganization constitutes an important level of transcriptional regulation in neurons.
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| http://dx.doi.org/10.1016/j.celrep.2022.110352 | DOI Listing |
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