The flavin adenine dinucleotide (FAD) is an indispensable coenzyme in live cells. It acts as a catalyst in many redox responsive metabolic reactions, including oxidative phosphorylation in mitochondria. The real-time monitoring of flavin is important to understand the disorder in the metabolic process, redox system, etc. Thus, we have developed a fluorescent probe that noncovalently binds with flavin to exhibit the FRET process. H- NMR and docking study indicated that there is a strong hydrophobic interaction between flavins and . Also, a π-π stacking between isoalloxazine ring in flavin and quinoline and coumarin moieties of favors self-assembly. The nontoxic probe could distinguish cancer cells from normal cells based on expressions of endogenous FAD.
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