Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy.

Mohit Jaiswal Trang T Tran Jiatong Guo Mingwei Zhou Josefina Garcia Diaz Gail E Fanucci Zhongwu Guo

Analyst

Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL 32611, USA.

Published: February 2022

A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885856	PMC
http://dx.doi.org/10.1039/d1an02226a	DOI Listing

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