AI Article Synopsis
- A common poultry pathogen has recently infected North American house finches after a host shift in 1994, but its virulence and host specificity are not well understood due to limited genomic tools.
- Researchers evaluated two recombination systems from related phages and successfully used the RecET-like system for gene inactivation and targeted replacements in the bacteria.
- The study also utilized the Cre-lox system to remove antibiotic resistance markers, marking the first effective genetic tool for engineering the pathogen's genome and showcasing the power of heterologous recombination systems in simpler bacteria.
Article Abstract
() is a common pathogen of poultry worldwide that has recently spread to North American house finches after a single host shift in 1994. The molecular determinants of virulence and host specificity are still largely unknown, mostly due to the absence of efficient methods for functional genomics. After evaluating two exogenous recombination systems derived from phages found in the phylogenetically related and the more distant , the RecET-like system from was successfully used for gene inactivation and targeted replacement in . In a second step, the Cre-lox recombination system was used for the removal of the antibiotic resistance marker in recombinant mutants. This study therefore describes the first genetic tool for targeted genome engineering of and demonstrates the efficiency of heterologous recombination systems in minimal bacteria.
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| http://dx.doi.org/10.1021/acssynbio.1c00541 | DOI Listing |
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