AI Article Synopsis
- Gene editing with CRISPR/Cas technology has transformed biotechnology, especially in medicine and life sciences, but its application to miRNAs in plants has been underexplored.
- This review discusses the development, creation, structure, and interactions of miRNAs, including how they regulate gene expression and techniques to predict their target genes.
- The integration of CRISPR/Cas with miRNAs in plant research will enhance our understanding of their functions and promote more sustainable and acceptable crop genetic engineering practices.
Article Abstract
Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.
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| http://dx.doi.org/10.1016/j.tplants.2022.01.009 | DOI Listing |
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