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Crystal Structure of H227A Mutant of Arginine Kinase in Suggests the Importance of Its Stability. | LitMetric
Arginine kinase (AK) plays a crucial role in the survival of , a water flea and a common planktonic invertebrate sensitive to water pollution, owing to the production of bioenergy. AK from (AK) has four highly conserved histidine residues, namely, H90, H227, H284, and H315 in the amino acid sequence. In contrast to AK WT (wild type), the enzyme activity of the H227A mutant decreases by 18%. To identify the structure-function relationship of this H227A mutant enzyme, the crystal 3D X-ray structure has been determined and an unfolding assay using anilino-1-naphthalenesulfonic acid (ANS) fluorescence has been undertaken. The results revealed that when compared to the AK WT, the hydrogen bonding between H227 and A135 was broken in the H227A crystal structure. This suggests that H227 residue, closed to the arginine binding site, plays an important role in maintaining the structural stability and maximizing the enzyme activity through hydrogen bonding with the backbone oxygen of A135.
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Jeonbuk National University , Iksan 54596, Korea.
Arginine kinase (AK) plays a crucial role in the survival of , a water flea and a common planktonic invertebrate sensitive to water pollution, owing to the production of bioenergy. AK from (AK) has four highly conserved histidine residues, namely, H90, H227, H284, and H315 in the amino acid sequence. In contrast to AK WT (wild type), the enzyme activity of the H227A mutant decreases by 18%.
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