Mining and design of biosensors for engineering microbial cell factory.

Improving the yield of target product is the purpose in the construction and optimization of microbial cell factory. Conferring biosensor-based intelligence on the strains is an important way to solve the engineering problems such as imbalance of metabolic flux and accumulation of toxic intermediates. In recent years, genetically encoded biosensors for both metabolites and environmental signals are rapidly created and developed via rational design, directed evolution or machine learning. In addition to being a rapid detection method for high-throughput screening, biosensors are more and more applied for directed evolution of strains and dynamic regulation of metabolic pathway. Biosensor-based intelligence will play more important role in the construction of microbial cell factory.