Cucurbitacin E (CuE) plays an important role in anticancer, antichemical carcinogenesis, and body immunity, etc., and the detection of its concentration is meaningful to pharmacological studies and clinical applications. However, the small molecular weight of CuE makes direct detection difficult through a surface plasmon resonance (SPR) sensor. In this work, we propose a cells-amplified signal strategy at the plasmonic interface, realizing the detection of CuE with ultra-low concentration. The seeded HeLa cells are modified onto the surface of the SPR sensor, and a small amount of CuE can lead to the remarkable morphology change of cells and the release of cell-related substances onto the plamonic interface, thus significantly amplifying the signal. Experimental results show that by using an unmodified SPR sensor with the bulk refractive index sensitivity of 2367.3 nm/RIU (RIU: refractive index unit), there no effective signal can be detected during the CuE concentration range of 0-100 nM; whereas, employing the proposed strategy, the signal for CuE detection can be significantly enhanced, resulting in a high detection sensitivity of 0.6196 nm/nM, corresponding to a limit of detection of 45.2 pM (25.2 pg/mL). The proposed cells-based signal amplifying strategy shows great potential applications in drug screening or bio-sensing to small molecules with low concentration.
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http://dx.doi.org/10.1364/BOE.445679 | DOI Listing |
Nano Lett
December 2024
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Guangzhou 510632, P. R. China.
Sensors (Basel)
December 2024
Department of Medical Diagnostics, Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02822 Warsaw, Poland.
Microvolume ELISA platforms have become vital in diagnostics for their high-throughput capabilities and minimal sample requirements. High-quality substrates with advanced surface properties are essential for these applications. They enable both efficient biomolecule immobilization and antifouling properties, which are critical for assay sensitivity and specificity.
View Article and Find Full Text PDFBiosens Bioelectron
November 2024
College of Information Science and Engineering, Northeastern University, Shenyang, 110819, China; State Key Laboratory of Synthetical Automation for Process Industries, Shenyang, 110819, China; Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Qinhuangdao, 066004, China. Electronic address:
Biosens Bioelectron
November 2024
Department of Optoelectronic Engineering, College of Physics and Optoelectronic Engineering, Jinan University, Guangzhou, 510632, PR China; Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Jinan University, Guangzhou, 510632, PR China; Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, PR China. Electronic address:
Surface plasmon resonance (SPR) optical fiber sensors are appealing for biomolecular detection due to their inherent characteristics such as flexibility, real-time performance, and high sensitivity. Concurrently, incorporating SPR sensors into wearable devices has emerged as a significant strategy. However, the majority of traditional SPR optical fiber sensors utilize spectrometers for optical readout, which leads to a relatively bulky overall size of the sensing system.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
State Key Laboratory of Metastable Materials Science & Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University, Qinhuangdao, 066004, PR China.
Background: Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on.
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