A simple acoustofluidic device for on-chip fabrication of PLGA nanoparticles.

Miniaturization of systems and processes provides numerous benefits in terms of cost, reproducibility, precision, minimized consumption of chemical reagents, and prevention of contamination. The field of microfluidics successfully finds a place in a plethora of applications, including on-chip nanoparticle synthesis. Compared with the bulk approaches, on-chip methods that are enabled by microfluidic devices offer better control of size and uniformity of fabricated nanoparticles. However, these microfluidic devices generally require complex and expensive fabrication facilities that are not readily available in low-resourced laboratories. Here, a low-cost and simple acoustic device is demonstrated by generating acoustic streaming flows inside glass capillaries through exciting different flexural modes. At distinct frequencies, the flexural modes of the capillary result in different oscillation profiles that can insert harmonic forcing into the fluid. We explored these flexural modes and identified the modes that can generate strong acoustic streaming vortices along the glass capillary. Then, we applied these modes for fluid mixing using an easy-to-fabricate acoustofluidic device architecture. This device is applied in the fabrication of poly(d,l-lactide--glycolide) (PLGA) nanoparticles. The acoustic device consists of a thin glass capillary and two polydimethylsiloxane adaptors that are formed using three-dimensional printed molds. By controlling the flow rates of the polymer and water solutions, PLGA nanoparticles with diameters between 65 and 96 nm are achieved with polydispersity index values ranging between 0.08 and 0.18. Owing to its simple design and minimal fabrication requirements, the proposed acoustofluidic mixer can be applied for microfluidic fluid mixing applications in limited resource settings.