Phage therapy, as an effective and specific method in the treatment of multidrug-resistant (MDR) bacterial infections, has attracted the attention of many researchers. In this study, a double-stranded DNA phage with the ability of lysing some strains of MDR (vB_Kpn_3) was isolated from hospitals' wastewater and then characterized morphologically and genetically. Transmission electron microscopy and genetic analyses have revealed that vB_Kpn_3 is a member of Siphoviridae family. One-step growth curve also showed a burst time of 35 min and a burst size of 31 PFU/ml. The genome of the phage is composed of 112,080 bp with 41.33% G + C content carrying 186 open reading frames. vB_Kpn_3 is a broad host range phage that infects MDR and some other species of Enterobacteriaceae such as and . In addition, no antibiotic resistance and toxin genes were detected in its genome.
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