This study evaluated the effects of combined OncoTherad immunotherapy and probiotic supplementation on colorectal carcinogenesis chemically induced with 1,2-dimethylhydrazine (DMH) in mice. The animals were randomly allocated in five groups: Control, DMH: did not receive any treatment; DMH + OncoTherad: received weekly I.P. (intraperitoneal) dose of OncoTherad; DMH + Probiotic: received daily administrations via gavage of the functional food (Lactobacillus: acidophilus and paracasei, Bifidobacterium: lactis and bifidum) and DMH + Probiotic + OncoTherad: received the same treatment than the previous groups. After ten weeks of treatment, the large intestine was collected for immunohistochemical analysis of TLR4, MyD88, NF-κB, IL-6, TLR2, TRIF, IRF-3, IFN-γ, Ki-67, KRAS, IL-10, and TGF-β. For the statistical analysis, the variance tests (ANOVA) and Kruskal-Wallis were used and significance set at p < 0.05. Probiotic supplementation associated with the OncoTherad were able to modulate weight loss, stimulate the canonical signaling pathway TLR2/TLR4 (MyD88-dependent), reduce the non-canonical signaling pathway (TRIF-dependent), attenuate the proliferative pathway mediated by Ki-67 and KRAS oncogene, and stimulate the production of IL-10 and TGF-β cytokines. Thus, the association of OncoTherad and probiotic supplementation has shown important immudomulatory effects and could be considered a potential new therapeutic approach for colorectal cancer after further investigations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.tice.2022.101747 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Probiotic Bacillus licheniformis DSMZ 28710 improves sow milk microbiota and enhances piglet health outcomes.
Sci Rep
January 2025
Multi-Omics for Functional Products in Food, Cosmetics and Animals Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand.
Maintaining a diverse and balanced sow milk microbiome is essential to piglet development. Thus, this study aimed to examine the effects of probiotic Bacillus licheniformis supplementation on the microbiome composition of sow colostrum and milk, and to review associated health findings in piglets. B.
View Article and Find Full Text PDFImpact of the probiotic on loperamide-induced delayed bowel movement in Sprague-Dawley rats.
Food Funct
January 2025
Institute of Human Behavior & Genetics, Korea University, Seoul 02841, Republic of Korea.
This study investigated the effects of on alleviating loperamide-induced constipation. To evaluate the efficacy of in Sprague-Dawley (SD) rats, fecal parameters, the intestinal transit rate, and changes in intestinal mucosal cells were measured through histological analysis. Additionally, serotonin levels, water absorption, tight junction-related gene expression, and the cecal short-chain fatty acid (SCFA) content were analyzed.
View Article and Find Full Text PDFRegulatory effects of HBUAS52074 on depression-like behavior induced by chronic social defeat stress in mice: modulation of the gut microbiota.
Food Funct
January 2025
Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.
The gut microbiome has emerged as a growing focus of research and public health interest, leading to the frequent exploration of probiotic dietary supplements as potential treatments for various disorders, such as anxiety and depression. In the present report, changes in inflammation and microbiome composition were assessed in model mice exhibiting depressive-like behaviors that were exposed to the probiotic HBUAS52074. It was found that HBUAS52074 alleviated the severity of depressive-like behaviors while increasing serum 5-HT concentrations.
View Article and Find Full Text PDFPreliminary report: Protective effects of probiotics on cefovecin-induced gut dysbiosis in dogs.
Can J Vet Res
January 2025
Laboratory of Veterinary Dermatology (Han, Hwang) and Research Institute for Veterinary Science (Han, Mun, Hwang), College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea; ORIGIN Veterinary Dermatology Clinic, Busan, Republic of Korea (Kang); Department of Companion Animal Health Care, College of Medical Health, Kyungbok University, Namyangju, Republic of Korea (Kim S-J); Department of Large Animal Internal Medicine, College of Veterinary Medicine, Kangwon National University, Chuncheon, Republic of Korea (Kim Y-H).
The objective of this study was to evaluate whether supplementation with probiotics over a 2-week period stabilizes the gut microbiota in dogs following prolonged cefovecin treatment. A significant number of clinical veterinarians prescribe oral probiotics to dogs in conjunction with systemic antibiotics with the intention of protecting against gut dysbiosis. The effects of antibiotics and probiotics in dogs have not been extensively studied, however, and the optimal treatment for gut dysbiosis remains uncertain.
View Article and Find Full Text PDFShort-term probiotic supplementation affects the diversity, genetics, growth, and interactions of the native gut microbiome.
Imeta
December 2024
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education Inner Mongolia Agricultural University Hohhot China.
Article Synopsis
- Probiotics can significantly enhance the diversity and genetic makeup of the gut microbiome, as shown by a study involving high-dose probiotics administered over a week to healthy individuals.
- Weekly fecal samples revealed adaptive changes in gut bacteria, indicating rapid evolutionary responses due to the introduction of probiotics.
- This research emphasizes the need for a deeper understanding of the ecological and evolutionary mechanisms behind probiotics to improve their application and effectiveness.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!