With concerns over global warming and climate change, many efforts have been devoted to mitigate atmospheric CO level. As a CO utilization strategy, formate dehydrogenase (FDH) from Clostridium species were explored to discover O-tolerant and efficient FDHs that can catalyze CO to formate (i.e. CO reductase). With FDH from Clostridium ljungdahlii (ClFDH) that plays as a CO reductase previously reported as the reference, FDH from C.autoethanogenum (CaFDH), C. coskatii (CcFDH), and C. ragsdalei (CrFDH) were newly discovered via genome-mining. The FDHs were expressed in Escherichia coli and the recombinant FDHs successfully catalyzed CO reduction with a specific activity of 15 U g-CaFDH, 17 U g-CcFDH, and 8.7 U g-CrFDH. Interestingly, all FDHs newly discovered retain their catalytic activity under aerobic condition, although Clostridium species are strict anaerobe. The results discussed herein can contribute to biocatalytic CO utilization.
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