AI Article Synopsis
- Ureaplasma urealyticum (UU) is a common bacteria in the human reproductive system that can cause infections, highlighting the need for quick detection methods.* -
- A new real-time fluorescence loop-mediated isothermal amplification (LAMP) assay was developed for detecting UU, using specific primers for ureaseB genes and monitored in a controlled temperature setup.* -
- The LAMP method showed high sensitivity and specificity, successfully detecting UU in all tested clinical samples, proving to be a better alternative to traditional PCR techniques.*
Article Abstract
Ureaplasma urealyticum (UU) is commonly present in human reproductive tract, which frequently leads to genital tract infection. Hence, there is an urgent need to develop a rapid detection method for UU. In our study, a real-time fluorescence loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of UU. Two primers were specifically designed based on the highly conserved regions of ureaseB genes. The reaction was carried out for 60 min in a constant temperature system using Bst DNA polymerase, and the process was monitored by real-time fluorescence signal, while polymerase chain reaction (PCR) was performed simultaneously. In real-time fluorescence LAMP reaction system, positive result was only obtained for UU among 9 bacterial strains, with detection sensitivity of 42 pg/μL (4.2 × 10 CFU/mL), and all 16 clinical samples of UU could be detected. In conclusion, real-time fluorescence LAMP is a simple, sensitive, specific and effective method compared with conventional PCR, which shows great promise in the rapid detection of UU.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837760 | PMC |
| http://dx.doi.org/10.1186/s13568-022-01357-2 | DOI Listing |
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