Lignocellulose is the most abundant biomass available on earth, including wood and agricultural wastes such as rice straw, corn cobs, and oil palm empty bunches. The biopolymer content in lignocellulose has a great potential as feedstock for producing industrial raw materials such as glucose, sorbitol, xylose, xylitol, and other pharmaceutical excipients. Currently, scientists and governments agree that the enzymatic delignification method is an environmentally friendly green method to be applied. This review attempts to explain the proper preparation of the enzymes laccase, lignin peroxidase, and manganese peroxidase, as well as the important factors influencing their activity. The recent applications of the enzymes for detoxification of hazardous substances, proper enzyme immobilization technique, and future prospect combination with DESs extraction of lignin are also discussed.
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http://dx.doi.org/10.1016/j.heliyon.2022.e08865 | DOI Listing |
J Gen Appl Microbiol
July 2024
Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Unidad Irapuato.
Bacteria represent an attractive source for the isolation and identification of potentially useful microorganisms for lignin depolymerization, a process required for the use of agricultural waste. In this work, ten autochthonous bacteria isolated from straw, cow manure, and composts were characterized for potential use in the biodelignification of the waste. A comparison of the ability to degrade lignin and the efficiency of ligninolytic enzymes was performed in bacteria grown in media with lignin as a sole carbon source (LLM, 3.
View Article and Find Full Text PDFInt J Mol Sci
November 2022
State Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Fungal laccases play important roles in the degradation of lignocellulose. In this study, the laccase producing cotton straw medium for was optimized by single-factor and orthogonal experiments, and to investigate the role of gene, one of the laccase-encoding genes, in the degradation of cotton straw lignin, an overexpression strain of gene was constructed, which was analyzed for the characteristics of lignin degradation. The results demonstrated that the culture conditions with the highest lignin degradation efficiency of the were the cotton straw particle size of 0.
View Article and Find Full Text PDFHeliyon
February 2022
Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia.
Lignocellulose is the most abundant biomass available on earth, including wood and agricultural wastes such as rice straw, corn cobs, and oil palm empty bunches. The biopolymer content in lignocellulose has a great potential as feedstock for producing industrial raw materials such as glucose, sorbitol, xylose, xylitol, and other pharmaceutical excipients. Currently, scientists and governments agree that the enzymatic delignification method is an environmentally friendly green method to be applied.
View Article and Find Full Text PDFMethods Enzymol
March 2022
Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States; Genetics Institute, University of Florida, Gainesville, FL, United States. Electronic address:
Haloarchaea and their enzymes have extremophilic properties desirable for use as platform organisms and biocatalysts in the bioindustry. These GRAS (generally regarded as safe) designated microbes thrive in hypersaline environments and use a salt-in strategy to maintain osmotic homeostasis. This unusual strategy has resulted in the evolution of most of the intracellular and extracellular enzymes of haloarchaea to be active and stable not only in high salt (2-5M) but also in low salt (0.
View Article and Find Full Text PDFData Brief
June 2021
Faculty of Engineering, Universitas Muhammadiyah Surakarta, Indonesia.
This article presents experimental data on oil palm biomass (oil palm leaves, oil palm trunk and empty fruit bunch) handsheet production characterization by biodelignification treatment using extracted from termite gut (). It associates the lignocellulose chemical composition obtained via technical association pulp and paper industry TAPPI T 222 om-02 testing on lignin content reduction determination, holocellulose and hemicellulose content determination (Kurscher-Hoffner method). Several data obtained for handsheet characterization presents brightness, opacity, contrast ratio, din transparency, thickness, bursting and tearing indexes are collected.
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