AI Article Synopsis
- The traditional gold nanoparticles (AuNPs) used in colorimetric lateral flow immunoassays (LFIA) are often limited in sensitivity due to their insufficient signal brightness.
- A new "three-in-one" multifunctional nanohybrid (FeO@MOF@Pt) has been developed, combining FeO nanoparticles, MIL-100(Fe), and platinum nanoparticles, which enhances colorimetric signal brightness and sensitivity.
- This new system can achieve a sensitivity of 0.5 pg/mL for procalcitonin testing, which is about 2280 times greater than conventional AuNP-based LFIA, indicating its potential for improved disease diagnostics.
Article Abstract
Colorimetric lateral flow immunoassay (LFIA) with gold nanoparticles (AuNPs) as signal reporters has been widely used in point-of-care testing. Nonetheless, the potential of traditional AuNP-based LFIA for the early diagnosis of disease is often compromised by limited sensitivity due to the insufficient colorimetric signal brightness of AuNPs. Herein, we develop a "three-in-one" multifunctional catalytic colorimetric nanohybrid (FeO@MOF@Pt) composed of FeO nanoparticles, MIL-100(Fe), and platinum (Pt) nanoparticles. FeO@MOF@Pt displays enhanced colorimetric signal brightness, fast magnetic response, and ultrahigh peroxidase-mimicking activity, which are beneficial to the enhancement of the sensitivity of LFIA by coupling with magnetic separation and catalytic amplification. When integrated with the dual-antibody sandwich LFIA platform, the developed FeO@MOF@Pt can achieve an ultrasensitive immunochromatographic assay of procalcitonin with a sensitivity of 0.5 pg mL, which is approximately 2280-fold higher than that of conventional AuNP-based LFIA and superior to previously published immunoassays. Therefore, this work suggests that the proposed catalytic colorimetric nanohybrid can act as promising signal reporters to enable ultrasensitive immunochromatographic disease diagnostics.
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| http://dx.doi.org/10.1021/acsnano.2c00008 | DOI Listing |
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