Background And Objectives: To evaluate the therapeutic potential of targeting highly differentiated T cells in patients with inclusion body myositis (IBM) by establishing high-resolution mapping of killer cell lectin-like receptor subfamily G member 1 (KLRG1) within the T and natural killer (NK) cell compartments.
Methods: Blood was collected from 51 patients with IBM and 19 healthy age-matched donors. Peripheral blood mononuclear cells were interrogated by flow cytometry using a 12-marker antibody panel. The panel allowed the delineation of naive T cells (Tn), central memory T cells (Tcm), 4 stages of effector memory differentiation T cells (Tem 1-4), and effector memory re-expressing CD45RA T cells (TemRA), as well as total and subpopulations of NK cells based on the differential expression of CD16 and C56.
Results: We found that a population of KLRG1 Tem and TemRA were expanded in both the CD4 and CD8 T-cell subpopulations in patients with IBM. KLRG1 expression in CD8 T cells increased with T-cell differentiation with the lowest levels of expression in Tn and highest in highly differentiated TemRA and CD56CD8 T cells. The frequency of KLRG1 total NK cells and subpopulations did not differ between patients with IBM and healthy donors. IBM disease duration correlated with increased CD8 T-cell differentiation.
Discussion: Our findings reveal that the selective expansion of blood KLRG1 T cells in patients with IBM is confined to the TemRA and Tem cellular compartments.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967422 | PMC |
| http://dx.doi.org/10.1212/WNL.0000000000200013 | DOI Listing |
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