AI Article Synopsis
- Enterovirus A71 (EV-A71) is a dangerous virus mainly affecting infants and children, causing severe neurological issues, and there are currently no antiviral drugs specifically for it, despite some vaccines.
- This study discovered a new antibody that can inhibit EV-A71 growth by targeting a host protein called α-enolase (ENO1), which was found more frequently in milder cases of EV-A71.
- Testing in a mouse model showed that this antibody significantly improved survival rates after viral infection and reduced virus levels and brain damage, suggesting its potential as a treatment for EV-A71.
Article Abstract
Background: Enterovirus A71 (EV-A71) is a neurotropic virus which may cause severe neural complications, especially in infants and children. The clinical manifestations include hand-foot-and-mouth disease, herpangina, brainstem encephalitis, pulmonary edema, and other severe neurological diseases. Although there are some vaccines approved, the post-marketing surveillance is still unavailable. In addition, there is no antiviral drugs against EV-A71 available.
Methods: In this study, we identified a novel antibody that could inhibit viral growth through a human single chain variable fragment (scFv) library expressed in mammalian cells and panned by infection with lethal dose of EV-A71.
Results: We identified that the host protein α-enolase (ENO1) is the target of this scFv, and anti-ENO1 antibody was found to be more in mild cases than severe EV-A71 cases. Furthermore, we examined the antiviral activity in a mouse model. We found that the treatment of the identified 07-human IgG antibody increased the survival rate after virus challenge, and significantly decreased the viral RNA and the level of neural pathology in brain tissue.
Conclusions: Collectively, through a promising intracellular scFv library expression and screening system, we found a potential scFv/antibody which targets host protein ENO1 and can interfere with the infection of EV-A71. The results indicate that the usage and application of this antibody may offer a potential treatment against EV-A71 infection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822709 | PMC |
| http://dx.doi.org/10.1186/s12929-022-00794-2 | DOI Listing |
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