AI Article Synopsis
Article Abstract
Transferable linezolid resistance due to , , and -like genes is increasingly detected in enterococci associated with animals and humans globally. We aimed to characterize the genetic environment of in linezolid-resistant isolates from Scotland. Six linezolid-resistant isolated from urogenital samples were confirmed to carry the gene by PCR. Short read (Illumina) sequencing showed the isolates were genetically distinct (>13900 core SNPs) and belonged to different MLST sequence types. Plasmid contents were examined using hybrid assembly of short and long read (Oxford Nanopore MinION) sequencing technologies. The gene was located on distinct plasmids in each isolate, suggesting that transfer of a single plasmid did not contribute to dissemination in this collection. pTM6294-2, BX5936-1 and pWE0438-1 were similar to -positive plasmids from China and Japan, while the remaining three plasmids had limited similarity to other published examples. We identified the novel Tn transposon in pWE0254-1 carrying linezolid (), macrolide () and spectinomycin [ANT(9)-Ia] resistance genes. OptrA amino acid sequences differed by 0-20 residues. We report multiple variants of on distinct plasmids in diverse strains of . It is important to identify the selection pressures driving the emergence and maintenance of resistance against linezolid to retain the clinical utility of this antibiotic.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941993 | PMC |
| http://dx.doi.org/10.1099/mic.0.001137 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bacteriological Profile of Patients With Stroke-Associated Pneumonia and Antimicrobial Susceptibility of Pathogens: A Cross-Sectional Study.
Cureus
November 2024
Microbiology, Kalinga Institute of Medical Sciences, Bhubaneswar, IND.
Background And Objectives: Stroke-associated pneumonia (SAP) is the aftermath of aspiration of oropharyngeal secretions or stomach content. Mechanical ventilation and lowered immunity and consciousness facilitate the etiopathogenesis of SAP. Antibiotic prophylaxis and repeated culture and sensitivity testing dampen the drug susceptibility patterns of the pathogens.
View Article and Find Full Text PDFThe gene is responsible for intrinsic resistance to various drugs and virulence in by regulating cell division.
Antimicrob Agents Chemother
December 2024
School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, China.
exhibits intrinsic resistance to most antibiotics, hence leading to infections that are difficult to treat. To address this issue, the identification of new molecular targets is essential for the development or repositioning of therapeutic agents. This study demonstrated that the -knockout strain, Mab, became significantly susceptible to a range of antibiotics, not only but also exhibited susceptibility to rifabutin, bedaquiline, and linezolid .
View Article and Find Full Text PDFresensitization of multidrug-resistant clinical isolates of and through phage-antibiotic synergy.
Antimicrob Agents Chemother
December 2024
Department of Pathology, University of California, San Diego, California, USA.
Bacteriophages are an increasingly attractive option for the treatment of antibiotic-resistant infections, but their efficacy is difficult to discern due to the confounding effects of antibiotics. Phages are generally delivered in conjunction with antibiotics, and thus, when patients improve, it is unclear whether the phages, antibiotics, or both are responsible. This question is particularly relevant for enterococcus infections, as limited data suggest phages might restore antibiotic efficacy against resistant strains.
View Article and Find Full Text PDFThe healthy human gut can take it all: vancomycin-variable, linezolid-resistant strains and specific bacteriocin-species interplay in spp.
Appl Environ Microbiol
December 2024
UCIBIO, Unidade de Ciências Biomoleculares Aplicadas, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
spp. are opportunistic human pathogens colonizing the human gut and a significant reservoir for the continuous adaptation of hospital clones. However, studies on the features of enterococci species co-colonizing healthy individuals are scarce.
View Article and Find Full Text PDFCombination of gallium citrate and levofloxacin induces a distinct metabolome profile and enhances growth inhibition of multidrug-resistant compared to linezolid.
Front Microbiol
November 2024
Department of Chemistry, Umeå, University, Umeå, Sweden.
Introduction: Tuberculosis (TB) treatment typically involves a tailored combination of four antibiotics based on the drug resistance profile of the infecting strain. The increasing drug resistance of () requires the development of novel antibiotics to ensure effective treatment regimens. Gallium (Ga) is being explored as a repurposed drug against TB due to its ability to inhibit growth and disrupt iron metabolism.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!