Orange-red to NIR emissive carbon dots for antimicrobial, bioimaging and bacteria diagnosis.

Antimicrobial photodynamic therapy (aPDT) has become a popular technology for the treatment of bacterial infections. The development of antimicrobial agents combining diagnosis and treatment remains a major challenge. Herein, curcumin carbon quantum dots (Cur-NRCQDs) with antibacterial and imaging effects were synthesized using a hydrothermal method. The fluorescence absorption range of the Cur-NRCQDs in aqueous solution was 555 to 850 nm, showing orange-red to near infrared (NIR) fluorescence, and its maximum emission wavelength was 635 nm. At the same time, Cur-NRCQDs improved the efficiency of Cur as the photosensitizer (PS), showed good storage and light stability, and enhanced the efficiency of reactive oxygen (ROS) generation and antibacterial activity. Under the irradiation of a xenon lamp, Cur-NRCQDs inactivated 100% () and () at concentrations of 10 and 15 μM, respectively. The possible reason for this was that under PDT, the ROS produced by the Cur-NRCQDs destroyed the integrity of the cell membrane, resulting in leakage of the contents. In addition, the Cur-NRCQDs showed good cell compatibility, as they can also enter bacteria and cells for imaging, so they can be employed for the detection of bacteria and cell tissues. Therefore, Cur-NRCQDs are an ideal candidate material for aPDT treatment and fluorescent bioimaging.