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TISSUE CLEARING. | LitMetric

TISSUE CLEARING.

Nat Rev Methods Primers

Harvard Center for Biological Imaging, Harvard University, Cambridge, MA, USA.

Published: December 2021

AI Article Synopsis

  • - Tissue clearing, which helps visualize biological samples in 3D, has recently gained popularity due to advances in optical physics and new microscopy techniques that can capture detailed images of large specimens.
  • - The Primer aims to simplify the complex literature surrounding tissue clearing, offering a modular approach that guides beginners in creating a customized clearing pipeline for their specific tissue samples.
  • - It also covers essential imaging and computational tools for large-scale tissue clearing, highlights current applications and limitations, and discusses potential future developments in the field.

Article Abstract

Tissue clearing of gross anatomical samples was first described over a century ago and has only recently found widespread use in the field of microscopy. This renaissance has been driven by the application of modern knowledge of optical physics and chemical engineering to the development of robust and reproducible clearing techniques, the arrival of new microscopes that can image large samples at cellular resolution and computing infrastructure able to store and analyze large data volumes. Many biological relationships between structure and function require investigation in three dimensions and tissue clearing therefore has the potential to enable broad discoveries in the biological sciences. Unfortunately, the current literature is complex and could confuse researchers looking to begin a clearing project. The goal of this Primer is to outline a modular approach to tissue clearing that allows a novice researcher to develop a customized clearing pipeline tailored to their tissue of interest. Further, the Primer outlines the required imaging and computational infrastructure needed to perform tissue clearing at scale, gives an overview of current applications, discusses limitations and provides an outlook on future advances in the field.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815095PMC
http://dx.doi.org/10.1038/s43586-021-00080-9DOI Listing

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