The therapeutic effect of IL-21 combined with IFN-γ inducing CD4CXCR5CD57T cells differentiation on hepatocellular carcinoma.

Introduction: Liver cancer is a malignant tumor with high incidence and short survival time. In order to increase the cure rate and disease-free survival rate of liver cancer, it is necessary to seek effective treatment methods.

Objectives: The objective of this study is to evaluate the therapeutic effects of IL-21 and IFN-γ inducing the formation of CD4CXCR5CD57T cells on liver cancer.

Methods: The methods of analyze the relationship between CD4CXCR5CD57T cells and the survival time of hepatocellular carcinoma (HCC), and study the effect of IL-21 combined with IFN-γ in inducing stem cells to differentiate into CD4CXCR5CD57T cells. The effects of IL-21 combined with IFN-γ induced CD4CXCR5CD57T cells on liver cancer were studied through animal experiments, and the regulatory mechanism, and the effect of hepatitis B virus (HBV) on it.

Results: The study found that the number of CD4CXCR5CD57T cells in serum of liver cancer patients with prolonged survival time increased significantly, the expression of CD4, CD57, and CXCR5 in the tumor microenvironment increased, and the serum IL-21 and IFN-γ concentrations increased. IL-21 and IFN-γ induce stem cells to differentiate into CD4CXCR5CD57T cells and induce HepG2 cells apoptosis. HBV leads to a decrease in the number of CD4CXCR5CD57T cells and a chronic inflammatory response. Treg cells can regulate CD4CXCR5CD57T cells. IL-21 combined with IFN-γ induced an increase in the number of CD4CXCR5CD57T cells in hepatocarcinoma-bearing mice, which has an inhibitory effect on H22 liver cancer.

Conclusion: The conclusion of the study is that IL-21 combined with IFN-γ induces stem cells to differentiate into CD4CXCR5CD57T cells, Treg can control the increase in their number, and HBV can cause their number to decrease, which can control the growth of liver cancer.