Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining the developmental stage-specific expression of genes of interest spatially and temporally. For complete details on the use and execution of this protocol, please refer to Watanabe-Takano et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792446 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101127 | DOI Listing |
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