AI Article Synopsis

  • Enhancer RNAs (eRNAs), specifically LINC02569, have been identified as important regulators in intervertebral disc degeneration (IDD), influencing cell functions and identity.
  • The research demonstrates that the novel siRNA delivery system (oCNT-pb) used for targeting LINC02569 significantly reduces inflammation and cellular aging in nucleus pulposus cells.
  • Overall, targeting eRNA LINC02569 shows promise as a therapeutic approach for treating IDD by modulating key signaling pathways.

Article Abstract

Enhancer RNAs (eRNAs) are noncoding RNAs that synthesized at active enhancers. eRNAs have important regulatory characteristics and appear to be significant for maintenance of cell identity and information processing. Series of functional eRNAs have been identified as potential therapeutic targets for multiple diseases. Nevertheless, the role of eRNAs on intervertebral disc degeneration (IDD) is still unknown yet. Herein, we utilized the nucleus pulposus samples of patients and identified a key eRNA (LINC02569) with the Arraystar eRNA Microarray. LINC02569 mostly locates in nucleus and plays an important role in the progress of IDD by activating nuclear factor kappa-B (NF-κB) signaling pathway. We used a cationic polymer brush coated carbon nanotube (oCNT-pb)-based siRNA delivery platform that we previously designed, to transport LINC02569 siRNA (si-02569) to nucleus pulposus cells. The siRNA loaded oCNT-pb accumulated in nucleus pulposus cells with lower toxicity and higher transfection efficiency, compared with the traditional siRNA delivery system. Moreover, the results showed that the delivery of si-02569 significantly alleviated the inflammatory response in the nucleus pulposus cells via inhibiting P65 phosphorylation and preventing its transfer into the nucleus, and meanwhile alleviated cell senescence by decreasing the expression of P21. Altogether, our results highlight that eRNA (LINC02569) plays important role in the progression of IDD and could be a potential therapeutic target for alleviation of IDD.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802762PMC
http://dx.doi.org/10.3389/fcell.2021.837777DOI Listing

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