We study the fusion of homogeneous cell aggregates and of hybrid aggregates combining cells and microparticles. In all cases, we find that the contact area does not vary linearly over time, as observed for liquid drops, but rather it follows a power law in . This result is interpreted by generalizing the fusion model of soft viscoelastic solid balls to viscoelastic liquid balls, akin to jelly pearls. We also explore the asymmetric fusion between a homogeneous aggregate and a hybrid aggregate. This latter experiment allows the determination of the self-diffusion coefficient of the cells in a tissue by following the spatial distribution of internalized particles in the cells.
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http://dx.doi.org/10.1021/acs.langmuir.1c02949 | DOI Listing |
Front Biosci (Landmark Ed)
January 2025
Department of Cardiovascular Medicine, Binzhou Medical University Hospital, 256603 Binzhou, Shandong, China.
Background: Cellular vacuolization is a commonly observed phenomenon under physiological and pathological conditions. However, the mechanisms underlying vacuole formation remain largely unresolved.
Methods: LysoTracker Deep Red probes and Enhanced Green Fluorescent Protein-tagged light chain 3B (LC3B) plasmids were employed to differentiate the types of massive vacuoles.
Materials (Basel)
January 2025
Department of Biomedical Engineering, Iwate Medical University, Iwate 028-3694, Japan.
This study aimed to investigate the release of metallic ions from cobalt-chromium (Co-Cr) alloys fabricated by additive manufacturing (AM) for comparison with dental casting. Co-Cr alloys were fabricated via AM using selective laser melting (SLM) and electron beam melting (EBM) in powder-bed fusion. Polished and mechanically ground specimens were prepared.
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January 2025
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.
Despite their high clinical relevance, obtaining structural and biophysical data on transmembrane proteins has been hindered by challenges involved in their expression and extraction in a homogeneous, functionally-active form. The inherent enzymatic activity of receptor tyrosine kinases (RTKs) presents additional challenges. Oncogenic fusions of RTKs with heterologous partners represent a particularly difficult-to-express protein subtype due to their high flexibility, aggregation propensity and the lack of a known method for extraction within the native lipid environment.
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January 2025
I-Form Advanced Manufacturing Research Centre, Dublin City University, Dublin, Ireland.
In the realm of materials science and engineering, the pursuit of advanced materials with tailored properties has been a driving goal behind technological progress. Scientific interest in laser powder bed fusion (L-PBF) fabricated NiTi alloy has in recent times seen an upsurge of activity. In this study, we investigate the impact of varying volume energy density (VED) during L-PBF on the microstructure and corrosion behaviour of NiTi alloys in both scan (XY) and built (XZ) planes.
View Article and Find Full Text PDFActa Neuropathol
January 2025
Center for Alzheimer's and Neurodegenerative Diseases, Peter O'Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Neurodegenerative tauopathies are characterized by the deposition of distinct fibrillar tau assemblies, whose rigid core structures correlate with defined neuropathological phenotypes. Essential tremor (ET) is a progressive neurological disorder that, in some cases, is associated with cognitive impairment and tau accumulation. In this study, we explored tau assembly conformation in ET patients with tau pathology using cytometry-based tau biosensor assays.
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