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Background: Human noroviruses are the major cause of acute gastroenteritis and exhibit considerable genetic diversity. Next generation sequencing (NGS) analysis based on environmental surveillance has been proved to be an effective method in norovirus surveillance.
Methods: Between January 2019 and December 2021, 36 sewage samples were collected and analyzed using real-time quantitative PCR to detect noroviruses.
Morphological and Phylogenetic Analyses of Ceratomyxa tessaloniensis n. sp. In Astyanax mexicanus De Fillipi, 1853 (Characiformes: Characidae) from the Flexal River, Tessalônica Community, Amapá, Amazon, Brazil.
Acta Parasitol
January 2025
Laboratory of Morpho-Molecular Integration and Technologies, Federal Rural University of the Amazon (UFRA), Belém, State of Pará, Brazil.
Purpose: This work described a new species of Ceratomyxa, based on morphological and phylogenetic analyzes of myxospores collected from the gallbladder of the fish Astyanax mexicanus.
Methods: Sixty-two specimens were captured, between December 2022 and February 2024, in the Flexal River, in the community of Tessalônica, state of Amapá. The specimens were transported alive to the Laboratory of Morphophysiology and Animal Health, at the State University of Amapá, where the studies were carried out.
Modelling the human Coenzyme Q deficiency in Drosophila melanogaster.
Free Radic Biol Med
January 2025
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC-JA, Sevilla, Spain; CIBERER, U729, Instituto de Salud Carlos III, Madrid, Spain. Electronic address:
The interference of the expression of each of the genes involved in the synthesis of coenzyme Q (CoQ) in Drosophila melanogaster can help to understand the pathophysiology of CoQ-dependent mitochondrial diseases in humans. We have knocked-down all genes involved in the CoQ biosynthesis pathway at different temperatures to induce depletion of CoQ at different levels throughout the body and in a tissue-specific manner. The efficiency of the knockdowns was quantified by Q-RTPCR and determination of CoQ levels by HPLC-UV+ECD.
View Article and Find Full Text PDFThe Genes of Nigerian Marek's Disease Virus (MDV) Field Isolates Contain Mutations Common to Both European and US High Virulence Strains.
Viruses
December 2024
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Background: Marek's disease (MD) is a pathology affecting chickens caused by Marek's disease virus (MDV), an acute transforming alphaherpesvirus of the genus . MD is characterized by paralysis, immune suppression, and the rapid formation of T-cell (primarily CD4+) lymphomas. Over the last 50 years, losses due to MDV infection have been controlled worldwide through vaccination; however, these live-attenuated vaccines are non-sterilizing and potentially contributed to the virulence evolution of MDV field strains.
View Article and Find Full Text PDFImage-Based Detection and Classification of Malaria Parasites and Leukocytes with Quality Assessment of Romanowsky-Stained Blood Smears.
Sensors (Basel)
January 2025
Space Robotics Research Group (SpaceR), Interdisciplinary Centre for Security, Reliability and Trust (SnT), University of Luxembourg, L-1855 Luxembourg, Luxembourg.
Malaria remains a global health concern, with 249 million cases and 608,000 deaths being reported by the WHO in 2022. Traditional diagnostic methods often struggle with inconsistent stain quality, lighting variations, and limited resources in endemic regions, making manual detection time-intensive and error-prone. This study introduces an automated system for analyzing Romanowsky-stained thick blood smears, focusing on image quality evaluation, leukocyte detection, and malaria parasite classification.
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