AI Article Synopsis
- Cell migration plays a crucial role in both normal processes like brain development and pathological issues such as cancer metastasis, with a focus on the involvement of the autophagy-linked FYVE protein (ALFY) in these contexts.
- ALFY has been found to localize to specific early endosomes during cell migration and is concentrated in the protrusions at both the front and back of migrating cells.
- The absence of ALFY leads to decreased cell attachment and improper integrin functioning, which disrupts the formation of the leading edge necessary for effective cell movement and directionality.
Article Abstract
Cell migration is a complex process underlying physiological and pathological processes such as brain development and cancer metastasis. The autophagy-linked FYVE protein (ALFY; also known as WDFY3), an autophagy adaptor protein known to promote clearance of protein aggregates, has been implicated in brain development and neural migration during cerebral cortical neurogenesis in mice. However, a specific role of ALFY in cell motility and extracellular matrix adhesion during migration has not been investigated. Here, we reveal a novel role for ALFY in the endocytic pathway and in cell migration. We show that ALFY localizes to RAB5- and EEA1-positive early endosomes in a PtdIns(3)P-dependent manner and is highly enriched in cellular protrusions at the leading and lagging edge of migrating cells. We find that cells lacking ALFY have reduced attachment and altered protein levels and glycosylation of integrins, resulting in the inability to form a proper leading edge and loss of directional cell motility.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919339 | PMC |
| http://dx.doi.org/10.1242/jcs.259138 | DOI Listing |
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