AI Article Synopsis
- Purkinje neurons (PNs) play a key role in motor learning and are impacted in conditions like spino-cerebellar ataxias.
- The text outlines a protocol to effectively measure calcium (Ca) transients in PNs from adult mice, which is crucial for assessing neuronal activity.
- The protocol also includes troubleshooting tips and references a detailed study by Dhanya and Hasan (2021) for further guidance.
Article Abstract
Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca transients can be challenging. Here, we describe a protocol for measuring Ca transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca signaling in cerebellar slices expressing a genetic Ca reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021).
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783150 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.101105 | DOI Listing |
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