Extracts Extend Lifespan and Alleviate Proteotoxicity in .

berry (Ningxia Gouqi, , goji berry, or wolfberry), as a traditional Chinese herb, was recorded beneficial for longevity in traditional Chinese medical scriptures and currently is a natural dietary supplement worldwide. However, under modern experimental conditions, the longevity effect of berry and the underlying mechanisms have been less studied. Here, we reported that total water extracts of berry (LBE), which contains 22% polysaccharides and other components, such as anthocyanins, extended the lifespan of without side effects on worm fertility and pharyngeal pumping. Interestingly, we found that the lifespan extension effect was more prominent in worms with shorter mean lifespan as compared to those with longer mean lifespan. Furthermore, we showed that the lifespan extension effect of LBE depended on deacetylase . Remarkably, LBE rescued heat shock transcription factor-1 () deficiency in wild-type worms with different mean lifespans, and this effect also depended on . In addition, we found that LBE extended lifespan and alleviated toxic protein aggregation in neurodegenerative worms with deficiency. Our study suggested that LBE may be a potential antiaging natural dietary supplement especially to individuals with malnutrition or chronic diseases and a potential therapeutic agent for neurodegenerative diseases characterized by deficiency.