AI Article Synopsis

  • The study investigates the effects of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression in transgenic rice on germination and early growth under varying salt levels.
  • The transgenic rice line (T5) with two copies of T-DNA demonstrated higher germination rates, enzyme activity, sugar accumulation, and chlorophyll production compared to non-transgenic counterparts in saline conditions.
  • Analysis revealed that the transgenic rice maintained porphyrin levels and showed enhanced morphological traits, indicating better salt tolerance linked to gene expression and thermal imaging results.

Article Abstract

encodes glyceraldehyde-3-phosphate dehydrogenase enzyme involved in sugar mobilization, particularly glycolysis and gluconeogenesis. The objective of this study was to determine physiological aspects of germination and early seedling establishment of ( glyceraldehyde-3-phosphate dehydrogenase) expressing transgenic rice (T5) against different salt concentrations. The T5 line that carried 2 copies of T-DNA and had the highest level of expression was used in the investigation. Final germination percentage, amylase activity, reducing sugar accumulation, and chlorophyll biosynthesis were comparatively higher in expressing transgenic rice against elevating saline conditions. A slow-paced conversion of porphyrin's precursors was seen through the matrix model and further elaborated by a graphical model. A sustained level of porphyrin was observed in expressing transgenic rice. These data were concurrent with the relative gene expression and thermal imaging (thermography) of expressing transgenic rice against salt stress. Morphological attributes also favored the salt tolerance exhibited by transformed rice.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790071PMC
http://dx.doi.org/10.3389/fpls.2021.767826DOI Listing

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