AI Article Synopsis
- This study explores the use of human hair as a method for assessing long-term exposure to mycotoxins, which are toxic compounds produced by fungi.
- The researchers developed a new analytical method that includes washing the hair and using enzymes to extract mycotoxins, followed by high-resolution mass spectrometry for accurate detection.
- Validating their method showed that 67% of tested hair samples contained at least one type of mycotoxin, including harmful aflatoxins, making it the first reliable technique for measuring multiple mycotoxins in hair.
Article Abstract
The potential of hair as matrix for assessing long-term exposure to mycotoxins remains scarcely explored. Therefore, this study aimed to develop and validate an analytical methodology for the simultaneous determination of aflatoxins, enniatins, beauvericin and T-2 toxin in human hair, based on a pretreatment stage prior to salt-assisted liquid-liquid extraction and followed by high performance liquid chromatography coupled to high resolution Q-TOF mass spectrometry for the first time. Washing with a non-ionic detergent was successfully applied, whereas enzymatic digestion with Pronase E was mandatory for releasing mycotoxins from the hair matrix. The methodology was validated according to Commission Decision 2002/657/EC, with limits of quantification ranging from 0.6 to 8.7 ng/g. The analysis of 10 samples showed at least one mycotoxin occurring in 67% of samples, including the carcinogenic aflatoxins. This is the first validated methodology for the quantification of multiple mycotoxins in human hair.
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| http://dx.doi.org/10.1016/j.jchromb.2022.123117 | DOI Listing |
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