AI Article Synopsis
- Researchers created a new tool for mapping molecular and elemental characteristics in the MeLiM model of melanoma using various mass spectrometry techniques.
- The study identified seven specific proteins associated with melanoma, including S100A12 and CD163, by utilizing MALDI-MSI and HPLC-MS/MS methods.
- Additionally, the ICP-MS technique was employed to detect the spatial presence of key elements like zinc and calcium, which are related to these melanoma-specific proteins.
Article Abstract
Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.
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| http://dx.doi.org/10.1016/j.ijbiomac.2022.01.139 | DOI Listing |
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