AI Article Synopsis
- Researchers are working on creating artificial cells that can mimic real biological processes by organizing different functions in distinct compartments.
- They developed a method to control molecular transport within these artificial cells using DNA-based coacervates that can be switched on or off with light.
- This approach shows potential for building complex artificial cells with multiple compartments that can communicate and work together effectively.
Article Abstract
Spatiotemporal organization of distinct biological processes in cytomimetic compartments is a crucial step towards engineering functional artificial cells. Mimicking controlled bi-directional molecular communication inside artificial cells remains a considerable challenge. Here we present photoswitchable molecular transport between programmable membraneless organelle-like DNA coacervates in a synthetic microcompartment. We use droplet microfluidics to fabricate membraneless non-fusing DNA coacervates by liquid-liquid phase separation in a water-in-oil droplet, and employ the interior DNA coacervates as artificial organelles to imitate intracellular communication via photo-regulated uni- and bi-directional transfer of biomolecules. Our results highlight a promising new route to assembly of multicompartment artificial cells with functional networks.
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| http://dx.doi.org/10.1002/anie.202117500 | DOI Listing |
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