has emerged as an important opportunistic pathogen worldwide, being responsible for large outbreaks for nosocomial infections, primarily in intensive care units. ATCC 19606 is the species type strain, and a reference organism in many laboratories due to its low virulence, amenability to genetic manipulation and extensive antibiotic susceptibility. We wondered if frequent propagation of ATCC 19606 in different laboratories may have driven micro- and macro-evolutionary events that could determine inter-laboratory differences of genome-based data. By combining Illumina MiSeq, MinION and Sanger technologies, we generated a high-quality whole-genome sequence of ATCC 19606, then performed a comparative genome analysis between ATCC 19606 strains from several research laboratories and a reference collection. Differences between publicly available ATCC 19606 genome sequences were observed, including SNPs, macro- and micro-deletions, and the uneven presence of a 52 kb prophage belonging to genus . Two plasmids, pMAC and p1ATCC19606, were invariably detected in all tested strains. The presence of a putative replicase, a replication origin containing four 22-mer direct repeats, and a toxin-antitoxin system implicated in plasmid stability were predicted by analysis of p1ATCC19606, and experimentally confirmed. This work refines the sequence, structure and functional annotation of the ATCC 19606 genome, and highlights some remarkable differences between domesticated strains, likely resulting from genetic drift.
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http://dx.doi.org/10.1099/mgen.0.000749 | DOI Listing |
Bioorg Med Chem
January 2025
Beijing Key Laboratory of Technology and Application for Anti-Infective New Drugs Research and Development, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:
Infectious diseases caused by drug-resistant bacteria represent one of the most significant global public challenges of this century. There is an urgent need for the treatment of drug-resistant Gram-negative bacterial infections. A series of 3,4-dihydro-2H-[1,3]oxazino[5,6-h]quinoline derivatives were synthesized and evaluated for their antibacterial activity against Gram-negative bacteria including strains from ATCC and clinical isolates, initially revealing the structure-activity relationship.
View Article and Find Full Text PDFFront Microbiol
December 2024
Department of Public Health, University of Naples Federico II, Naples, Italy.
Introduction: The persistence of in the contaminated environment is sustained by tolerance to biocides and ability to growth as biofilm. The aim of the study was to analyze the susceptibility of biofilms to chlorhexidine (CHX) and benzalkonium (BZK) biocides and the ability of natural monomeric stilbenoid resveratrol (RV) to modulate the phenomenon.
Methods: Biofilm formation and preformed biofilm were tested by Crystal violet and tetrazolium salt reduction assay, respectively.
Can J Microbiol
December 2024
Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, Wenzhou, Zhejiang Province, China.
The objective of this study was to compare chlorhexidine digluconate and other antibiotics susceptibility of four species of the complex, and further investigate the chlorhexidine digluconate (CHG) tolerance mechanisms and molecular epidemic characteristics. Of 889 complex isolates, , and accounted for 84.2%, 10.
View Article and Find Full Text PDFJ Microbiol
November 2024
Department of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang- Ro, Jung-gu, Daegu, 41944, Republic of Korea.
Acinetobacter baumannii is a multidrug-resistant opportunistic pathogen primarily associated with hospital-acquired infections. The bacterium can gain multidrug resistance through several mechanisms, including horizontal gene transfer. A CRISPR-Cas system including several Cas genes could restrict the horizontal gene transfer.
View Article and Find Full Text PDFSci Rep
November 2024
Monash Biomedicine Discovery Institute, Department of Pharmacology, Monash University, Clayton, VIC, 3800, Australia.
This untargeted metabolomics study investigated the synergistic antibacterial activity of polymyxin B and Leu-teixobactin, a depsipeptide inhibitor of cell wall biosynthesis. Checkerboard microdilution assays revealed a significant synergy against polymyxin-susceptible and -resistant A. baumannii, excluding lipopolysaccharide-deficient variants.
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