Cryo-ultramicrotomy and Mass Spectrometry Imaging Analysis of Nudibranch Microstructures.

In this paper, we investigate the presence of latrunculin A in the outer rim of a nudibranch and show that by combining ultrathin cryosection methods with MALDI MSI we can achieve improved lateral ( and ) resolution and very high resolution in the dimension by virtue of the ultrathin 200 nm thin cryosections. We also demonstrate that a post ionization laser increases sensitivity. Recent advances in MALDI source design have improved the lateral resolution ( and ) and sensitivity during MSI. Taken together, very high resolution, from ultrathin sections, and improved lateral ( and ) resolution will allow for subcellular molecular imaging with the potential for subcellular 3D volume reconstruction.