[Correlation between TOPA2A gene expression and the number of CD4 T cells in hepatocellular carcinoma and its clinical prognostic significance].

Objective To analyze the correlation between the expression of TOP2A gene and the proportion of CD4T cells in hepatocellular carcinoma (HCC) and its clinical prognostic significance. Methods The expression of TOP2A mRNA in normal liver tissues and HCC tissues and its significance for survival and prognosis of HCC patients were analyzed by BioGPS, GEPIA and Kaplan-Meier Plotter databases. The coexpression gene of TOP2A and its GO function were analyzed using GENE and Metascape databases, along with the KEGG pathway enrichment analysis. The correlation between TOP2A and microsatellite instability (MSI) and DNA repair gene was analyzed by Sangerbox database. Then, the correlation between TOP2A gene and CD4 T cells and various immune cells was analyzed by TISIDB and TIMER database, and analysis was also performed regarding the effect of CD4 T cells on the survival and prognosis of HCC patients. Results TOP2A mRNA is not significantly expressed in normal liver tissues and CD4 T cells, but is significantly expressed in HCC tissue, which is not conducive to the survival and prognosis of patients. The GO function of TOP2A coexpression gene is mainly enriched in cell mitosis and cell proliferation, while KEGG is mainly enriched in cell cycle and platinum drug resistance pathway. The expression of TOP2A is positively correlated with MSI, MSH2 and MSH6 of DNA repair gene, the purity of tumor cells and the numbers of various immune cells. All kinds of immune cells reported certain copy number variation in HCC, but only the numbers of CD4 T cells showed a significant effect on the survival and prognosis of HCC patients. Conclusion There is a significant positive correlation between the expression of TOP2A mRNA and the number of CD4T cells in HCC, which is not conducive to the survival and prognosis of HCC patients.