Background: Osbeckia octandra is a plant endemic to Sri Lanka and is used in ethnomedicine for treating various diseases. However, the anti-cancer properties of O. octandra are yet to be fully investigated. In the present study, we evaluated the anti-cancer effects of O. octandra on oral cancer cells.
Methods: Human oral cancer cell lines (HSC2, YD10B, YD38, YD9, and YD32) were used in this study. BrdU incorporation, cell cycle and annexin-V/PI staining were all evaluated using flow cytometry to determine the extent to which O. octandra leaf extract inhibits cell proliferation and induces apoptosis. Cell viability and reactive oxygen species (ROS) were also measured in order to investigate the anti-cancer effects of O. octandra extracts. Western blotting was performed to detect cell cycle related protein such as cyclin d1 and cdk4, and to detect apoptosis-related proteins such as Bcl-2, Bcl-X, Bax, Caspase-9, Cleaved caspase-3, Fas, Caspase-8, and Bid.
Results: Leaf extract of O. octandra reduced oral squamous cell carcinoma (OSCC) cell viability in a dose-dependent manner. Leaf extract of O. octandra has non-toxic in normal keratinocytes. Also, O. octandra extract interrupted the DNA replication via G1 phase arrests, and this effect was independent of ROS generation. In the apoptosis-related experiments, the population of annexin V-positive cells increased upon treatment with O. octandra extract. Furthermore, the expression of anti-apoptotic protein (Bcl-2 and Bcl-xL) was decreased, whereas the expression of cleaved caspase-3 protein was increased in O. octandra-treated OSCC cells.
Conclusions: The results suggest that a leaf extract of O. octandra inhibited the proliferation of OSCC cells through G phase arrest and interrupting DNA replication. The leaf extract of O. octandra could trigger the apoptotic response via caspase 3 activation in OSCC cells. These results suggest that O. octandra has the potential to be developed as an alternative medicine for treating OSCC.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787916 | PMC |
| http://dx.doi.org/10.1186/s12906-022-03505-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Persimmon (Diospyros kaki L.) leaves accelerates skin tissue regeneration in excisional wound model: possible molecular mechanisms.
J Mol Histol
January 2025
Department of Chemical Engineering, Toronto Metropolitan University, Toronto, ON, Canada.
Persimmon (Diospyros kaki L.) leaves are a traditional medicinal herb used for treating many infectious and inflammatory-related conditions, including wound healing. To validate its traditional use, our study evaluates the acute toxicity and wound-healing effects of methanolic extracts of Persimmon (Diospyros kaki L.
View Article and Find Full Text PDFEnhancement of the production of terpenoid and flavonoid secondary metabolites in the ground and aerial parts of licorice composite plant in a hydroponic system.
J Biotechnol
January 2025
Biotechnology Research Department, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), National Botanical Garden, Tehran Karaj Freeway, P.O. Box 13185-116, Tehran, Iran.
Hairy roots mediated by Agrobacterium rhizogenes can be obtained from the composite plants (plants with hairy roots and untransformed aerial parts) by ex vitro method. Composite plants can produce higher amounts of secondary metabolites by merging hydroponic systems. This provides a stable condition for composite plants, in which various metabolites are produced in different parts.
View Article and Find Full Text PDFExploring biomass derived microcrystalline cellulose from the waste aquatic plant Pistia stratiotes: A comprehensive characterization for polymer composite reinforcement.
Int J Biol Macromol
January 2025
School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea. Electronic address:
The creation of polymer composites with better performance is a crucial thing. The cellulosic filler material gain popularity in polymer composites. In this study, aquatic plant Pistia stratiote leaves were used as a raw material for cellulose extraction.
View Article and Find Full Text PDFPrediction of ADMET profile and anti-inflammatory potential of chamuangone.
Sci Rep
January 2025
Faculty of Medical Technology, Prince of Songkla University, Songkhla, 90110, Thailand.
Chamuangone, a compound extracted from the leaves of Garcinia cowa, exhibits various biological activities. Yet, its absorption, distribution, metabolism, excretion, and toxicity (ADMET) profile as well as anti-inflammatory effects in macrophages remain unexplored. In this study, we employed a computational tool to predict the ADMET profile of chamuangone and performed molecular docking simulations to assess its interactions with key proteins involved in inflammatory pathways.
View Article and Find Full Text PDFFundamental Understanding of Bio-Nano Interface of Lysozyme on Polyphenol Coated Silver Nanoparticles: Mechanistic Insights into the Effect of Protein Corona on the Antibacterial Efficacy.
Langmuir
January 2025
Department of Chemical and Biological Sciences, National Institute of Technology Meghalaya, Shillong 793003, India.
Recent times have witnessed revolutionary progress in the design and development of functionalized nanomaterials as promising tools for biomedicinal applications. However, the gap in the fundamental understanding of the "biological responses" of the nanomaterials after the formation of "protein-corona" when it is exposed to the body system has drawn a thin line from its discoveries to real clinical trial. In this article we have synthesized two different silver NPs capped with the polyphenols of (guava) leaf extract and the other with one of its major polyphenolic groups, morin.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!