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One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system. | LitMetric

One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system.

Protein Expr Purif

Research Group Neuroplasticity, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany; Center for Behavioral Brain Sciences, Otto von Guericke University, 39120, Magdeburg, Germany; Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany; German Center for Neurodegenerative Diseases (DZNE), 39120, Magdeburg, Germany.

Published: May 2022

AI Article Synopsis

Article Abstract

Lamin B1 is an intermediate filament protein that is a core component of the nuclear lamina. Structural studies and biochemical characterization of lamin B1 are severely hampered by the tendency of the protein to form inclusion bodies in E. coli bacterial expression systems. Therefore, the purity and consistency of the protein varies from batch to batch. In this work, we have purified a tag-free lamin B1 protein from a soluble fraction following bacterial expression. We also checked the functional properties of the purified as well as of the subsequently lyophilised protein. The current protocol helps to purify functional lamin B1 in a single step.

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Source
http://dx.doi.org/10.1016/j.pep.2022.106057DOI Listing

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