Serum neurofilament light concentration does not increase following exposure to low velocity football heading.

: To investigate if heading frequency and impact biomechanics in a single session influence the concentration of serum neurofilament light (NF-L), a sensitive biomarker for axonal damage, up to 7 days after heading incident at ball velocities reflecting basic training drills.: Forty-four males were randomized into either control ( = 8), 10 header ( = 12), 20 header ( = 12) or 40 header ( = 12) groups. Linear and angular head accelerations were quantified during heading. Venous blood samples were taken at baseline, 6 h, 24 h and 7 days after heading. Serum NF-L was quantified using Quanterix NF-L assay kit on the Simoa HD-1 Platform.: Serum NF-L did not alter over time ( = 0.44) and was not influenced by number of headers [ = 0.47; mean (95% CI) concentrations at baseline 6.00 pg · ml (5.00-7.00 pg · ml); 6 h post 6.50 pg · ml (5.70-7.29 pg · ml); 24 h post 6.07 pg · ml (5.14-7.01 pg · ml); and 7 days post 6.46 pg · ml (5.45-7.46 pg · ml)]. There was no relationship between percentage change in NF-L and summed session linear and angular head accelerations.: In adult men, heading frequency or impact biomechanics did not affect NF-L response during a single session of headers at ball velocities reflective of basic training tasks.