AI Article Synopsis
- Triphenylamine (TP) derivatives, particularly TP-2Bzim, show potential as fluorescent probes for two-photon imaging and mitochondrial labeling in live cells.
- Two new TP-2Bzim derivatives were developed, demonstrating different localization in cells: one moved from mitochondria to lysosomes, while the other retained mitochondrial labeling and good photophysical properties.
- The study explored how calcium channels affect the uptake of the TP-Br probe and demonstrated the effectiveness of SXRF imaging for detecting the probe in live cells, highlighting its specificity for certain organelles.
Article Abstract
Triphenylamine (TP) derivatives such as two-branch cationic vinylbenzimidazolium triphenylamine TP-2Bzim are promising turn-on fluorescent probes suitable for two-photon imaging, labelling mitochondria in live cells. Here, we designed two TP-2Bzim derivatives as bimodal probes suitable for X-ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP-RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP-Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP-Br was studied. Synchrotron Radiation X-ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP-Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging.
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| http://dx.doi.org/10.1002/chem.202104424 | DOI Listing |
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