A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionpj720edvvqssjvfv2bisgflaha1tpibt): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 994
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3134
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Genetic and Chemical Diversity of Edible Mushroom Species. | LitMetric

Genetic and Chemical Diversity of Edible Mushroom Species.

Biomed Res Int

College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.

Published: April 2022

AI Article Synopsis

  • The study focused on a widely cultivated edible mushroom genus, evaluating genetic diversity among 132 samples using three molecular characteristics, revealing five distinct clades through phylogenetic analysis with solid statistical support.
  • 94 polymorphic DNA fragments were identified using intersimple sequence repeat (ISSR) markers, with specific differences noted between cultivated and wild strains, highlighting the distinct banding profiles.
  • The chemical composition analysis showed significant homogeneity among samples, revealing common sugars, amino acids, polar lipids, and the presence of menaquinone-6, suggesting the method used is effective for differentiating species within the genus and assessing genetic diversity.

Article Abstract

The genus is one of the most widely cultivated and edible mushrooms with various cultivators. Three molecular characteristics were used to evaluate the genetic diversity of 132 tested samples. Phylogenetic analysis showed five clades for tested samples of the genus by the combined ITS and LSU sequences with strong bootstraps and Bayesian posterior probability supports. A total of 94 polymorphic fragments ranging from 10 to 100 bp were observed by using an intersimple sequence repeat (ISSR) marker. The DNA fragment pattern showed that cultivator (strain P9) was clearly distinguished from wild strain based on their clear banding profiles produced. DNA GC content of the genus varied from 55.6 mol% to 43.3 mol%. Their chemical composition was also determined, including sugar, amino acid, polar lipid, mycolic acid, quinone, and fatty acid, which presented some high homogeneity. Most of the tested samples contained mycolic acid; glucose and arabinose as the main sugars; aspartic acid, arginine, lysine, tyrosine, and alanine as the main amino acids; and C, C, C -9,12, -C, and summed feature 8 as the main fatty acids. In addition, their polar lipid profiles were investigated for the first time, which significantly varied among species. The genus contained menaquinone-6 as the sole respiratory quinone, which showed a significant difference with that of its closely related genera. These results of this study demonstrated that the combined method above could efficiently differentiate each species and thus be considered an efficient tool for surveying the genetic diversity of the genus .

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783721PMC
http://dx.doi.org/10.1155/2022/6068185DOI Listing

Publication Analysis

Top Keywords

tested samples
12
species genus
8
genetic diversity
8
polar lipid
8
mycolic acid
8
genus
5
acid
5
genetic chemical
4
chemical diversity
4
diversity edible
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!