spp. have higher fitness for survival, in a pH-dependent manner, in pancreatic juice among duodenal bacterial flora.

Background And Aim: Bacterial infection is involved in the progression of many gastrointestinal diseases, including those of pancreas; however, how and which bacteria colonize in pancreatic juice and tissue have yet to be elucidated. Recently, we reported that exists in the pancreatic juice and tissues of patients with chronic pancreatic disease. Here, we investigated the survival of in duodenal juice with different pH conditions.

Methods: Pancreatic juice samples from 62 patients with cancers of the duodeno-pancreato-biliary region were evaluated for the presence of . 16S ribosomal RNA polymerase chain reaction and 16S-based metagenome analyses were performed to determine the bacterial composition. The survival of in various pancreatic juice conditions was evaluated.

Results: Of 62 samples, 27% (17/62) were positive for spp., among which 71% (12/17) contained . spp. showed the highest fitness for survival in alkaline pancreatic juice among various bacterial species. The microbiome of pancreatic juice from patients with pancreatic and bile duct cancer showed diversity, but spp. were enriched among duodenal tumors and intraductal papillary mucinous neoplasms.

Conclusions: Alkalinity is one of the important factors for the selective survival of among microbiota. can colonize the pancreatic duct when the pancreatic juice condition is altered.