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| http://dx.doi.org/10.21037/atm-21-2923 | DOI Listing |
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Enhancing pathogens detection in suspected geriatric bloodstream infections using Nanopore-targeted sequencing.
Microbiol Spectr
November 2024
Department of Clinical Laboratory,, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, China.
Unlabelled: Bloodstream infections (BSIs) are a critical concern for elderly patients, where prompt and accurate diagnosis is vital for effective treatment. Traditional blood culture methods suffer from delayed results and susceptibility to false-negatives. Nanopore-targeted sequencing (NTS) offers rapid pathogen detection and reporting, presenting a promising alternative.
View Article and Find Full Text PDFNanopore-targeted sequencing (NTS) for intracranial tuberculosis: a promising and reliable approach.
Ann Clin Microbiol Antimicrob
October 2024
Department of Tuberculosis, The Second Hospital of Nanjing, Nanjing, 211100, China.
Article Synopsis
- The World Health Organization estimated 10.6 million new tuberculosis cases and 1.5 million deaths in 2022, highlighting the need for effective diagnostics, especially for challenging conditions like tuberculous meningitis.
- A study with 122 patients at a hospital in Nanjing compared a new sequencing technology called Nanopore-targeted sequencing (NTS) with traditional methods (Xpert, MTB culture, PCR, AFB smear) for diagnosing intracranial tuberculosis using cerebrospinal fluid samples.
- NTS showed a sensitivity of 60.0% and a specificity of 95.5%, outperforming other methods, and identified significantly higher risks for patients testing positive for the disease compared to negative results.
High-throughput nanopore targeted sequencing for efficient drug resistance assay of .
Front Microbiol
May 2024
School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Drug-resistant tuberculosis (TB), especially multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB), is one of the urgent clinical problems and public health challenges. Culture-based phenotypic drug susceptibility testing (pDST) is time-consuming, and PCR-based assays are limited to hotspot mutations. In this study, we developed and validated a convenient and efficient approach based on high-throughput nanopore sequencing technology combined with multiplex PCR, namely nanopore targeted sequencing (NTS), to simultaneously sequence 18 genes associated with antibiotic resistance in (MTB).
View Article and Find Full Text PDFClinical application of nanopore-targeted sequencing technology in bronchoalveolar lavage fluid from patients with pulmonary infections.
Microbiol Spectr
June 2024
Department of Infectious Diseases, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.
The rapid and effective identification of pathogens in patients with pulmonary infections has posed a persistent challenge in medicine, with conventional microbiological tests (CMTs) proving time-consuming and less sensitive, hindering early diagnosis of respiratory infections. While there has been some research on the clinical performance of targeted sequencing technologies, limited focus has been directed toward bronchoalveolar lavage fluid (BALF). This study primarily evaluates the pathogen detection capabilities of nanopore-targeted sequencing (NTS) in BALF, providing a comprehensive analysis.
View Article and Find Full Text PDFMolecular rapid diagnostic testing for bloodstream infections: Nanopore targeted sequencing with pathogen-specific primers.
J Infect
June 2024
Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China; Zhejiang Key Laboratory of Clinical In Vitro Diagnostic Techniques, Hangzhou, Zhejiang 310003, China; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China. Electronic address:
Background: Nanopore sequencing, known for real-time analysis, shows promise for rapid clinical infection diagnosis but lacks effective assays for bloodstream infections (BSIs).
Methods: We prospectively assessed the performance of a novel nanopore targeted sequencing (NTS) assay in identifying pathogens and predicting antibiotic resistance in BSIs, analyzing 387 blood samples from December 2021 to April 2023.
Results: The positivity rate for NTS (69.
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