Microbial production of chemicals is a more sustainable alternative to traditional chemical processes. However, the shift to bioprocess is usually accompanied by a drop in economic feasibility. Co-production of more than one chemical can improve the economy of bioprocesses, enhance carbon utilization and also ensure better exploitation of resources. While a number of tools exist for metabolic engineering, there is a dearth of computational tools that can co-optimize the production of multiple metabolites. In this work, we propose co-FSEOF (co-production using Flux Scanning based on Enforced Objective Flux), an algorithm designed to identify intervention strategies to co-optimize the production of a set of metabolites. Co-FSEOF can be used to identify all pairs of products that can be co-optimized with ease using a single intervention. Beyond this, it can also identify higher-order intervention strategies for a given set of metabolites. We have employed this tool on the genome-scale metabolic models of and , and identified intervention targets that can co-optimize the production of pairs of metabolites under both aerobic and anaerobic conditions. Anaerobic conditions were found to support the co-production of a higher number of metabolites when compared to aerobic conditions in both organisms. The proposed computational framework will enhance the ease of study of metabolite co-production and thereby aid the design of better bioprocesses.
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http://dx.doi.org/10.3389/fbioe.2021.779405 | DOI Listing |
Multi-frequency acousto-optic beam splitting plays a significant role in many laser applications due to its advantages of inertia free, fast refreshing speed and high-precision controllable light intensity and direction. How to reduce the intermodulation distortion and improve the uniformity of the beam splitting intensity has become an intensive issue. This paper proposes the collaborative optimization method to co-optimize of the phase and amplitude of acousto-optic device multi-frequency composite signals, which can eliminate the nonlinear distortion of the amplification of multi-frequency composite signals and increase the energy utilization rate and accuracy of intensity control for each sub-beam.
View Article and Find Full Text PDFNat Food
January 2024
College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China.
Gels
March 2023
Department of Food Science and Technology, International Hellenic University, Alexander Campus, 57400 Thessaloniki, Greece.
Pectin and polyphenols have been obtained from choice peach flesh using microwave extraction, with the resulting extracts used in functionalizing strained yogurt gels. A Box-Behnken design was utilized in order to co-optimize the extraction process. Soluble solid content, total phenolic content, and particle size distributions were measured in the extracts.
View Article and Find Full Text PDFMAbs
January 2023
Centre for Misfolding Diseases, Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
Antibody drugs should exhibit not only high-binding affinity for their target antigens but also favorable physicochemical drug-like properties. Such drug-like biophysical properties are essential for the successful development of antibody drug products. The traditional approaches used in antibody drug development require significant experimentation to produce, optimize, and characterize many candidates.
View Article and Find Full Text PDFFront Plant Sci
April 2022
DIADE Group, Cereal Root Systems, Institute de Recherche pour le Développement/Université de Montpellier, Montpellier, France.
Selection criteria that co-optimize water use efficiency and yield are needed to promote plant productivity in increasingly challenging and variable drought scenarios, particularly dryland cereals in the semi-arid tropics. Optimizing water use efficiency and yield fundamentally involves transpiration dynamics, where restriction of maximum transpiration rate helps to avoid early crop failure, while maximizing grain filling. Transpiration restriction can be regulated by multiple mechanisms and involves cross-organ coordination.
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