Malassezia (M.) genus includes commensal yeasts of increasing medical importance, as they result in many diseases, ranging from pityriasis versicolor (PV) to systemic infections. Previous studies reported geographical variations in distribution of Malassezia species in PV lesions. The aims of the current study were to define the clinico-demographic features of PV in Tunisia, to characterize Malassezia isolates using phenotypic and molecular techniques and to find out any association between species and clinico-demographic parameters. In total, 120 PV patients were enrolled in this study. Skin scrapings were collected and inoculated on Sabouraud agar and modified Dixon medium. Malassezia species were identified using conventional phenotypic methods and 26 s rDNA PCR-RFLP. The highest prevalence of PV was observed among young adults' group. The most affected body areas were the back and neck. In overall, 50.8% and 35% of PV cases had pruritus and history of recurrence respectively. The overall concordance between phenotypic and molecular methods was high (80.95%). The discordant results are rather due to the presence of multiple species in a single culture than true misidentification. Using PCR-RFLP, M. furfur was the most isolated species (38.7%) followed by M. globosa (37.7%), M. restricta and M. sympodialis. No statistically significant association was noted between Malassezia spp. and clinico-demographic characteristics. Unlike many reports from temperate climate countries, M. furfur and M. globosa along together were the most frequently isolated species in Tunisian PV patients. Although phenotypic methods remain simple and cost-effective, molecular techniques are considered as fast and accurate methods for diagnosis purposes.
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http://dx.doi.org/10.1016/j.mycmed.2022.101246 | DOI Listing |
Mycoses
December 2024
Department of Dermato-Venereology, 4 Th Military Hospital, Wroclaw, Poland.
Background: Superficial fungal infections (SFI) are contagious conditions affecting the skin and its appendages, caused by various fungal species. Monitoring the distribution of common pathogens and identifying at-risk patient groups are essential for effective management and prevention.
Objectives: This study investigates the characteristics of SFI in Poland's Malopolska region from 2017 to 2019, focusing on etiological agents, infection sites and risk factors.
Indian J Dermatol
October 2024
From the Department of Medicine, Division of Dermatology, National University Hospital Singapore, Singapore, 5 Lower Kent Ridge Rd, Singapore.
Background: Superficial cutaneous fungal infections are common dermatologic conditions. A significant proportion do not present with typical clinical findings. However, the 10% potassium hydroxide (KOH) smear, a simple bedside test, is often underused when diagnosing cutaneous fungal infections.
View Article and Find Full Text PDFFront Med (Lausanne)
November 2024
Translational Medicine Institute, Semmelweis University, Budapest, Hungary.
Background: Colonization of the human gut and tumor tissue by non-pathogenic fungi has emerged as a potential risk factor associated with cancer epidemics. Therefore, we aimed to conduct a systematic review to assess the role of fungal colonization in gastrointestinal (GI) tumors in increasing diagnostic efficiency.
Methods: A PubMed citation search was conducted for publications up to and including March 2023, followed by full-text screening.
Med Mycol
December 2024
Aix-Marseille Université, SSA, AP-HM, RITMES, Marseille, France.
Malassezia species are lipid-dependent yeasts of the normal skin mycobiota in humans and some animals. Yet, both the dynamic of Malassezia skin colonization and the associated fungal and bacterial skin microbiome remain unknown in HIV+ patients. This study aimed to compare Malassezia yeast community structure and associated microbiome on the healthy skin of HIV+ patients and healthy controls.
View Article and Find Full Text PDFFront Microbiol
November 2024
Department of Gastroenterology, The Second Affiliated Hospital of Hainan Medical University, Haikou, China.
Objective: This study investigates the differential impact of fecal fungal microbiota on the pathogenesis of alcohol-associated liver disease (ALD) and metabolic-associated fatty liver disease (MAFLD). We aim to delineate distinct microbial patterns across various stages of each disease.
Methods: We conducted fungal internal transcribed spacer 2 (ITS2) sequencing analysis on fecal samples from 48 ALD patients, 55 MAFLD patients, and 64 healthy controls (HCs).
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