Impact of reduced uterine perfusion pressure model of preeclampsia on metabolism of placenta, maternal and fetal hearts.

Sci Rep

Centre for Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London, EC1M 6BQ, UK.

Published: January 2022

Preeclampsia is a cardiovascular pregnancy complication characterised by new onset hypertension and organ damage or intrauterine growth restriction. It is one of the leading causes of maternal and fetal mortality in pregnancy globally. Short of pre-term delivery of the fetus and placenta, treatment options are limited. Consequently, preeclampsia leads to increased cardiovascular disease risk in both mothers and offspring later in life. Here we aim to examine the impact of the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia on the maternal cardiovascular system, placental and fetal heart metabolism. The surgical RUPP model was induced in pregnant rats by applying silver clips around the aorta and uterine arteries on gestational day 14, resulting in ~ 40% uterine blood flow reduction. The experiment was terminated on gestational day 19 and metabolomic profile of placentae, maternal and fetal hearts analysed using high-resolution H NMR spectroscopy. Impairment of uterine perfusion in RUPP rats caused placental and cardiac hypoxia and a series of metabolic adaptations: altered energetics, carbohydrate, lipid and amino acid metabolism of placentae and maternal hearts. Comparatively, the fetal metabolic phenotype was mildly affected. Nevertheless, long-term effects of these changes in both mothers and the offspring should be investigated further in the future.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782944PMC
http://dx.doi.org/10.1038/s41598-022-05120-2DOI Listing

Publication Analysis

Top Keywords

uterine perfusion
12
maternal fetal
12
impact reduced
8
reduced uterine
8
perfusion pressure
8
model preeclampsia
8
fetal hearts
8
mothers offspring
8
gestational day
8
placentae maternal
8

Similar Publications

Article Synopsis
  • Uterus transplantation is currently the only solution for women with absolute uterine infertility, such as those with Rokitansky syndrome, allowing them to experience pregnancy and childbirth.
  • There are significant challenges with uterus transplantation, including the risks of ischemia-reperfusion injury and a notable thrombotic complication rate of up to 20%, which can lead to graft rejection.
  • This protocol aims to outline the surgical steps for obtaining porcine uteruses and implementing dynamic preservation through machine perfusion, which may reduce hypoxic injury compared to traditional static cold storage methods.
View Article and Find Full Text PDF
Article Synopsis
  • The study assesses a novel MRI radiomics stacking ensemble learning model for predicting outcomes of high-intensity focused ultrasound (HIFU) ablation in patients with uterine fibroids.
  • Data from 360 patients is analyzed, utilizing deep learning for automated segmentation and various statistical methods for feature selection to enhance predictive accuracy.
  • Results showed that among the models tested, the Multilayer Perceptron (MLP) performed the best with an area under the curve (AUC) of 0.858, indicating strong potential for preoperative prognosis using MRI features.
View Article and Find Full Text PDF

Toll-like receptor 4 inhibition by pyridostigmine is associated with a reduction in hypertension and inflammation in rat models of preeclampsia.

J Hypertens

February 2025

Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, People's Republic of China.

Background: Preeclampsia (PE) is marked by hypertension and detrimental sterile inflammatory response. Despite the reported anti-inflammatory effect of pyridostigmine bromide (PYR) in different models, its anti-inflammatory mechanism in PE is unclear. This study assessed whether such an anti-inflammatory effect involves inhibition of placental Toll-like receptor 4 (TLR4) signaling.

View Article and Find Full Text PDF

Background: Uterus transplantation from deceased donors offers a promising solution to the organ shortage, but optimal preservation methods are crucial for successful outcomes. Our primary objective is to conduct an initial assessment of the contribution of oxygenated hypothermic perfusion in uterine transplantation.

Methods: We performed a preclinical study on a porcine model of controlled donation after circulatory death (60 min warm ischemia).

View Article and Find Full Text PDF
Article Synopsis
  • Patients with thin endometrium often face difficulties with pregnancy during frozen-thawed embryo transfers; enhancing endometrial receptivity may help.
  • A study involving 150 patients tested the effects of intrauterine perfusion of granulocyte colony-stimulating factor (G-CSF) and human chorionic gonadotropin (HCG) on pregnancy outcomes, comparing results among three groups (control, HCG, and G-CSF).
  • The G-CSF group showed significant improvements in endometrial thickness, blood flow, and Treg levels, resulting in higher pregnancy rates than both the HCG and control groups, indicating G-CSF's potential benefit for patients with thin endometrium.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!