Histidinibacterium aquaticum sp. nov., Isolated from Salt-Field Sea Water.

A Gram stain-negative, aerobic, motile, and rod-shaped bacterium designated 176SS1-4 was isolated from the salt-field sea water. Strain 176SS1-4 grew well at 25 °C on marine agar. The taxonomic affiliation of the novel isolate was identified using the polyphasic approach. By comparative 16S rRNA gene sequence, it could be shown that strain 176SS1-4 branches within the family Rhodobacteraceae and class Alphaproteobacteria and is related to Histidinibacterium lentulum B17 (96.0% sequence similarity). The digital DNA-DNA hybridization and average nucleotide identity between strain 176SS1-4 and H. lentulum B17 was 19.2% and 77%. The genome comprises of 3,905,029 bp with a G + C content of 67.7 mol. Ubiquinone 10 (Q-10) was the major respiratory quinone. The major fatty acids were summed feature 8 (comprising C ω7c and/or C ω6c), C, C cyclo ω8c, C, and C1 ω6c 11-methyl and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylcholine. On the basis of the genotypic and phenotypic characteristics, strain 176SS1-4 can be placed as a novel species within the genus Histidinibacterium; the name Histidinibacterium aquaticum sp. nov. has been proposed, with type strain 176SS1-4 (= KACC 19891 = LMG 31032).