AI Article Synopsis
- Fluorescence nanoscopy has revolutionized life sciences by achieving 20-30 nm resolution with far-field fluorescence microscopes, though this limit is due to the photostability of fluorophores.
- Newer techniques in fluorescence nanoscopy are being developed to achieve resolutions under 10 nm, allowing for the visualization of structural proteins at a molecular level.
- The review highlights essential principles of these techniques, examines critical experimental factors for optimal performance, and considers current challenges and future prospects in the field.
Article Abstract
Fluorescence nanoscopy represented a breakthrough for the life sciences as it delivers 20-30 nm resolution using far-field fluorescence microscopes. This resolution limit is not fundamental but imposed by the limited photostability of fluorophores under ambient conditions. This has motivated the development of a second generation of fluorescence nanoscopy methods that aim to deliver sub-10 nm resolution, reaching the typical size of structural proteins and thus providing true molecular resolution. In this review, we present common fundamental aspects of these nanoscopies, discuss the key experimental factors that are necessary to fully exploit their capabilities, and discuss their current and future challenges.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724505 | PMC |
| http://dx.doi.org/10.1007/s12551-021-00864-z | DOI Listing |
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