Evaluation of Optimal Reference Genes for qRT-PCR Analysis in (Drury).

Insects

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.

Published: January 2022

The relative quantification of gene expression is mainly achieved through reverse transcription-quantitative PCR (qRT-PCR); however, its reliability and precision rely on proper data normalization using one or more optimal reference genes. (Drury) has been an invasive pest of forest trees, ornamental plants, and fruit trees in China for many years. Currently, the molecular physiological role of reference genes in is unclear, which hinders functional gene study. Therefore, eight common reference genes, , , , , , , , , were selected to evaluate levels of gene expression stability when subjected to varied experimental conditions, including developmental stage and gender, different tissues, larvae reared on different hosts and different larval density. The geNorm, BestKeeper, ΔCt method, and NormFinder statistical algorithms were used to normalize gene transcription data. Furthermore, the stability/suitability of these candidates was ranked overall by RefFinder. This study provides a comprehensive evaluation of reference genes in and could help select reference genes for other Lepidoptera species.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778541PMC
http://dx.doi.org/10.3390/insects13010097DOI Listing

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