Genes that influence the growth of Pacific abalone () may improve the productivity of the aquaculture industry. Previous research demonstrated that the differential expression of a gene encoding a C-type lectin domain-containing protein (CTLD) was associated with a faster growth in Pacific abalone. We analyzed this gene and identified an open reading frame that consisted of 145 amino acids. The sequence showed a significant homology to other genes that encode CTLDs in the genus . Expression profiling analysis at different developmental stages and from various tissues showed that the gene was first expressed at approximately 50 days after fertilization (shell length of 2.47 ± 0.13 mm). In adult Pacific abalone, the gene was strongly expressed in the epipodium, gill, and mantle. Recombinant Pacific abalone CTLD purified from exhibited antimicrobial activity against several Gram-positive bacteria (, , and ) and Gram-negative bacteria ( and ). We also performed bacterial agglutination assays in the presence of Ca, as well as bacterial binding assays in the presence of the detergent dodecyl maltoside. Incubation with . and . cells suggested that the CTLD stimulated Ca-dependent bacterial agglutination. Our results suggest that this novel Pacific abalone CTLD is important for the pathogen recognition in the gastropod host defense mechanism.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775961PMC
http://dx.doi.org/10.3390/ijms23020698DOI Listing

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